Hotspot

trnS-GCU-trnG-UCC

LSC · 8695-9276 · 582 bp

Back to Hoya

Pi 0.0277
MarkerSeek score 74.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
582
variable_sites
85
pi
0.0277
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 623-1159 bp 1.0 63.4
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA AACGAATCACACTTTTACCACT 45.0 36.4 58.0 56.2 624-1160 bp 1.0 61.5
trnS-GCU-trnG-UCC_p3 GCTTTAGTCCACTCAGCCAT ACGAATCACACTTTTACCACT 50.0 38.1 57.6 55.5 609-1145 bp 1.0 60.4
trnS-GCU-trnG-UCC_p4 ATTAGCAATCCGCCGCTTTA TACCACTAAACTATACCCGC 45.0 45.0 58.0 54.0 609-1145 bp 1.0 59.6
trnS-GCU-trnG-UCC_p5 AGTCCACTCAGCCATCTCTC ACGAATCACACTTTTACCACT 55.0 38.1 58.5 55.5 604-1140 bp 1.0 59.6