Hotspot

trnQ-UUG-psbK

LSC · 7533-8019 · 487 bp

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Pi 0.0187
MarkerSeek score 70.70
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
487
variable_sites
72
pi
0.0187
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GACCAAAACCCGTTGCCTTA AAAAAGCGTAGGCCTCGG 50.0 55.6 58.7 57.7 621-695 bp 1.0 68.2
trnQ-UUG-psbK_p2 AAAACCCGTTGCCTTACCAC AAAAAGCGTAGGCCTCGG 50.0 55.6 59.0 57.7 617-691 bp 1.0 65.9
trnQ-UUG-psbK_p3 ACCAAAACCCGTTGCCTTAC AAAAAGCGTAGGCCTCGG 50.0 55.6 59.0 57.7 620-694 bp 1.0 65.9
trnQ-UUG-psbK_p4 CAAAACCCGTTGCCTTACCA AAAAAGCGTAGGCCTCGG 50.0 55.6 59.0 57.7 618-692 bp 1.0 65.9
trnQ-UUG-psbK_p5 AAACCCGTTGCCTTACCAC AAAAAGCGTAGGCCTCGG 52.6 55.6 58.3 57.7 616-690 bp 1.0 63.4