Hotspot

rps16-trnQ-UUG

LSC · 6154-7460 · 1307 bp

Back to Hoya

Pi 0.0199
MarkerSeek score 71.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1307
variable_sites
151
pi
0.0199
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCTACTCGAGCTCCATCGT AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.0 58.1 1735-2341 bp 1.0 91.2
rps16-trnQ-UUG_p2 TTCTACTCGAGCTCCATCGT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1740-2346 bp 1.0 91.2
rps16-trnQ-UUG_p3 AACTTGCTCGAATGGGATCC AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.0 58.1 1851-2457 bp 0.962963 89.7
rps16-trnQ-UUG_p4 AACTTGCTCGAATGGGATCC CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 1856-2462 bp 0.962963 89.7
rps16-trnQ-UUG_p5 GATTCCCGCGTGATACACTT AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.1 58.1 1921-2527 bp 0.962963 89.6