Hotspot

accD-psaI

LSC · 89798-90699 · 902 bp

Back to Hovenia

Pi 0.0037
MarkerSeek score 52.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
902
variable_sites
5
pi
0.0037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTCCATGCTTTCTTTCCTT AGAAACCATTGCAATTGCCG 45.0 45.0 56.3 57.9 1027-1047 bp 1.0 68.2
accD-psaI_p2 GCTCCATGCTTTCTTTCCTT CCGGAAAAACTAAGCCCACT 45.0 50.0 56.3 58.1 1010-1030 bp 1.0 68.1
accD-psaI_p3 GCTCCATGCTTTCTTTCCTT CCATTGCAATTGCCGGAAAA 45.0 45.0 56.3 58.2 1022-1042 bp 1.0 67.3
accD-psaI_p4 GCTCCATGCTTTCTTTCCTTT AGAAACCATTGCAATTGCCG 42.9 45.0 57.1 57.9 1027-1047 bp 1.0 66.2
accD-psaI_p5 GCTCCATGCTTTCTTTCCTTT CCGGAAAAACTAAGCCCACT 42.9 50.0 57.1 58.1 1010-1030 bp 1.0 66.1