Hotspot

rps4-trnT-UGU

LSC · 76233-76862 · 630 bp

Back to Hovenia

Pi 0.00317

MarkerSeek score 53.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 3
pi: 0.00317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	725-790 bp	1.0	83.1
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	729-794 bp	1.0	82.7
rps4-trnT-UGU_p3	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	677-742 bp	1.0	82.4
rps4-trnT-UGU_p4	TAAGATCGCTTCCGGCTCTA	GGTTCGATTCCGATAGCTGG	50.0	55.0	58.0	58.2	684-749 bp	1.0	82.4
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	693-758 bp	1.0	82.2