Hotspot

trnE-UUC-trnT-GGU

LSC · 60092-60781 · 690 bp

Back to Hovenia

Pi 0.00193
MarkerSeek score 60.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
690
variable_sites
2
pi
0.00193
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CATAGTACCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.0 58.0 826-828 bp 1.0 80.1
trnE-UUC-trnT-GGU_p2 CATAGTACCCTACCCCCAGG TACCGCTGAGTTAAAAGGGC 60.0 50.0 58.0 57.9 791-793 bp 1.0 79.4
trnE-UUC-trnT-GGU_p3 CATAGTACCCTACCCCCAGG CCGATGACTTACGCCTTACC 60.0 55.0 58.0 58.2 823-825 bp 1.0 79.0
trnE-UUC-trnT-GGU_p4 CATAGTACCCTACCCCCAGG AAACCAAAGCCCCTTATCGG 60.0 50.0 58.0 58.2 850-852 bp 1.0 78.9
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 791-793 bp 1.0 78.2