Hotspot

atpA-atpH

LSC · 39740-41542 · 1803 bp

Back to Hovenia

Pi 0.00074

MarkerSeek score 59.00

Primer available yes

Label atpA-atpH

Feature Metadata

label_name: atpA-atpH
feature_type: igs
region: LSC
length_bp: 1803
variable_sites: 2
pi: 0.00074
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpH_p1	ACTTGGAGTACAGTACCGGT	GTTGTGGCATTAGCGCTTTT	50.0	45.0	57.7	57.9	1947-1949 bp	1.0	77.4
atpA-atpH_p2	TGCCATCACCTACTTGGAGT	GTTGTGGCATTAGCGCTTTT	50.0	45.0	58.3	57.9	1958-1960 bp	1.0	77.1
atpA-atpH_p3	ACTTGGAGTACAGTACCGGT	TGGACTGGTTGTGGCATTAG	50.0	50.0	57.7	57.8	1954-1956 bp	1.0	76.3
atpA-atpH_p4	TTGCTCAATACGTTCACGGA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	57.6	57.9	1900-1902 bp	1.0	76.3
atpA-atpH_p5	TGCCATCACCTACTTGGAGT	TGGACTGGTTGTGGCATTAG	50.0	50.0	58.3	57.8	1965-1967 bp	1.0	76.0