Hotspot

rps16-trnQ-UUG

LSC · 32892-33814 · 923 bp

Back to Hovenia

Pi 0.00144
MarkerSeek score 49.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
923
variable_sites
2
pi
0.00144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GTCGAATAGAAATGGGGCGT 50.0 50.0 57.9 58.1 1073-1084 bp 1.0 80.2
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG TCGAATAGAAATGGGGCGTG 50.0 50.0 57.9 58.1 1072-1083 bp 1.0 80.2
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1013-1024 bp 1.0 80.1
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GTCGAATAGAAATGGGGCGT 45.0 50.0 58.1 58.1 1056-1067 bp 1.0 79.8
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TCGAATAGAAATGGGGCGTG 45.0 50.0 58.1 58.1 1055-1066 bp 1.0 79.8