primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	rpl2	1	TGATCATTACGCTTCACCCG	ACGTGTCACAATTAATTACTCCT	20	23	50.0	34.8	58.1	56.0	0.0	0.0	5.110529	24865	26385	1657-1657	1.000000	44.0
rpl2_p2	rpl2	2	TGATCATTACGCTTCACCCG	AATGATTCGCTACAAAAGGA	20	20	50.0	35.0	58.1	52.9	0.0	0.0	5.179791	24865	26385	1691-1691	1.000000	44.0
rpl2_p3	rpl2	3	TGATCATTACGCTTCACCCG	TGATTCGCTACAAAAGGATT	20	20	50.0	35.0	58.1	52.9	0.0	0.0	5.179791	24865	26385	1689-1689	1.000000	44.0
rpl2_p4	rpl2	4	TGATCATTACGCTTCACCCG	TGAACGTGTCACAATTAATTACTCC	20	25	50.0	36.0	58.1	57.9	0.0	0.0	5.189759	24865	26385	1660-1660	1.000000	44.0
rpl2_p5	rpl2	5	TGATCATTACGCTTCACCCG	TGATTCGCTACAAAAGGATTT	20	21	50.0	33.3	58.1	53.8	0.0	0.0	5.261304	24865	26385	1689-1689	1.000000	44.0
matK-trnK-UUU_p1	matK-trnK-UUU	1	CCACGATCATGAGCAAATGC	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	57.9	58.2	12.1	17.1	0.283768	30151	30900	880-883	1.000000	78.0
matK-trnK-UUU_p2	matK-trnK-UUU	2	CCACGATCATGAGCAAATGC	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	57.9	58.2	12.1	17.1	0.283768	30151	30900	879-882	1.000000	78.0
matK-trnK-UUU_p3	matK-trnK-UUU	3	CCACGATCATGAGCAAATGC	GGGTTGCTAACTCAACGGTA	20	20	50.0	50.0	57.9	57.5	12.1	0.0	0.576105	30151	30900	893-896	1.000000	75.7
matK-trnK-UUU_p4	matK-trnK-UUU	4	CCACGATCATGAGCAAATGC	TTCGGGTTGCTAACTCAACG	20	20	50.0	50.0	57.9	58.5	12.1	0.0	0.610989	30151	30900	896-899	1.000000	75.4
matK-trnK-UUU_p5	matK-trnK-UUU	5	AGGAGATCGTGTTGCTGAGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.4	58.2	0.0	17.1	0.618093	30151	30900	828-831	1.000000	75.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GTCGAATAGAAATGGGGCGT	20	20	50.0	50.0	57.9	58.1	7.8	0.0	0.130821	32892	33814	1073-1084	1.000000	80.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	TCGAATAGAAATGGGGCGTG	20	20	50.0	50.0	57.9	58.1	7.8	0.0	0.132182	32892	33814	1072-1083	1.000000	80.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	32892	33814	1013-1024	1.000000	80.1
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	GTCGAATAGAAATGGGGCGT	20	20	45.0	50.0	58.1	58.1	8.1	0.0	0.189859	32892	33814	1056-1067	1.000000	79.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TCGAATAGAAATGGGGCGTG	20	20	45.0	50.0	58.1	58.1	8.1	0.0	0.191220	32892	33814	1055-1066	1.000000	79.8
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	34900	37655	2827-2863	1.000000	66.1
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	34900	37655	2826-2862	1.000000	66.1
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	34900	37655	2833-2869	1.000000	65.5
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	34900	37655	2832-2868	1.000000	65.5
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.2	56.9	0.0	0.0	2.202953	34900	37655	2881-2917	1.000000	64.9
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GTTGGAATGAAAAGCGTCCA	AGGAACAGCTGGAACGTTTT	20	20	45.0	45.0	56.9	57.9	0.0	19.9	1.130527	37728	38215	614-690	1.000000	72.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GTTGGAATGAAAAGCGTCCA	GAGGAAGCAGAAGCCCTTTT	20	20	45.0	50.0	56.9	58.1	0.0	0.0	1.161239	37728	38215	648-724	1.000000	71.7
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GTTGGAATGAAAAGCGTCCA	TTTACCGAGGAAGCAGAAGC	20	20	45.0	50.0	56.9	57.9	0.0	0.0	1.172507	37728	38215	654-730	1.000000	71.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GTTGGAATGAAAAGCGTCCA	ATTCAGGAACAGCTGGAACG	20	20	45.0	50.0	56.9	58.2	0.0	19.9	1.264231	37728	38215	618-694	1.000000	70.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AGTTGGAATGAAAAGCGTCC	AGGAACAGCTGGAACGTTTT	20	20	45.0	45.0	56.6	57.9	0.0	19.9	1.426573	37728	38215	615-691	1.000000	69.6
atpA-atpH_p1	atpA-atpH	1	ACTTGGAGTACAGTACCGGT	GTTGTGGCATTAGCGCTTTT	20	20	50.0	45.0	57.7	57.9	14.0	18.7	0.363899	39740	41542	1947-1949	1.000000	77.4
atpA-atpH_p2	atpA-atpH	2	TGCCATCACCTACTTGGAGT	GTTGTGGCATTAGCGCTTTT	20	20	50.0	45.0	58.3	57.9	0.0	18.7	0.407986	39740	41542	1958-1960	1.000000	77.1
atpA-atpH_p3	atpA-atpH	3	ACTTGGAGTACAGTACCGGT	TGGACTGGTTGTGGCATTAG	20	20	50.0	50.0	57.7	57.8	14.0	0.0	0.498153	39740	41542	1954-1956	1.000000	76.3
atpA-atpH_p4	atpA-atpH	4	TTGCTCAATACGTTCACGGA	GTTGTGGCATTAGCGCTTTT	20	20	45.0	45.0	57.6	57.9	4.1	18.7	0.506785	39740	41542	1900-1902	1.000000	76.3
atpA-atpH_p5	atpA-atpH	5	TGCCATCACCTACTTGGAGT	TGGACTGGTTGTGGCATTAG	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.542240	39740	41542	1965-1967	1.000000	76.0
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.056966	41789	42857	1157-1161	1.000000	80.6
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	41789	42857	1123-1127	1.000000	80.5
atpH-atpI_p3	atpH-atpI	3	GATCCCTTCTACAGCTTGGC	GCGAATCCATGGAAGGTCAT	20	20	55.0	50.0	58.0	58.0	0.0	20.7	0.066711	41789	42857	1208-1212	1.000000	80.5
atpH-atpI_p4	atpH-atpI	4	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	41789	42857	1187-1191	1.000000	79.7
atpH-atpI_p5	atpH-atpI	5	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	41789	42857	1153-1157	1.000000	79.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.0	58.0	0.0	0.0	0.031606	60092	60781	826-828	1.000000	80.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CATAGTACCCTACCCCCAGG	TACCGCTGAGTTAAAAGGGC	20	20	60.0	50.0	58.0	57.9	0.0	0.0	0.129173	60092	60781	791-793	1.000000	79.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	20	20	60.0	55.0	58.0	58.2	0.0	0.0	0.176539	60092	60781	823-825	1.000000	79.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CATAGTACCCTACCCCCAGG	AAACCAAAGCCCCTTATCGG	20	20	60.0	50.0	58.0	58.2	0.0	0.0	0.178307	60092	60781	850-852	1.000000	78.9
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	60092	60781	791-793	1.000000	78.2
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.155684	76233	76862	725-790	1.000000	83.1
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.196808	76233	76862	729-794	1.000000	82.7
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.236277	76233	76862	677-742	1.000000	82.4
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TAAGATCGCTTCCGGCTCTA	GGTTCGATTCCGATAGCTGG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.238419	76233	76862	684-749	1.000000	82.4
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263437	76233	76862	693-758	1.000000	82.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	20	20	55.0	50.0	58.0	57.7	10.3	5.0	0.350916	78888	79579	843-844	1.000000	78.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	20	20	50.0	50.0	58.1	57.7	0.0	5.0	0.400307	78888	79579	807-808	1.000000	78.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	20	20	55.0	50.0	57.9	57.7	2.5	5.0	0.484709	78888	79579	831-832	1.000000	77.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GTCGTGAATGGTCGGGATAG	ATGCCGGAAAGTTGGATAGG	20	20	55.0	50.0	57.9	57.7	0.0	5.0	0.484075	78888	79579	856-857	1.000000	77.7
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GAGGATGATGCGTCGTGAAT	ATGCCGGAAAGTTGGATAGG	20	20	50.0	50.0	58.1	57.7	5.1	5.0	0.490319	78888	79579	867-868	1.000000	77.6
atpB-rbcL_p1	atpB-rbcL	1	AATACGCCCCAGGTTGTTTT	AACACCGGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	85012	85893	1002-1041	1.000000	77.9
atpB-rbcL_p2	atpB-rbcL	2	AATACGCCCCAGGTTGTTTT	ACCGGCTTTGAATCCAACAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	85012	85893	999-1038	1.000000	77.9
atpB-rbcL_p3	atpB-rbcL	3	ATACGCCCCAGGTTGTTTTT	AACACCGGCTTTGAATCCAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	85012	85893	1001-1040	1.000000	77.9
atpB-rbcL_p4	atpB-rbcL	4	ATACGCCCCAGGTTGTTTTT	ACCGGCTTTGAATCCAACAA	20	20	45.0	45.0	58.0	58.3	0.0	0.0	0.305431	85012	85893	998-1037	1.000000	77.9
atpB-rbcL_p5	atpB-rbcL	5	CGGTGGAAAAGCTACATCCA	ACCGGCTTTGAATCCAACAA	20	20	50.0	45.0	57.9	58.3	10.2	0.0	0.409444	85012	85893	1041-1080	1.000000	77.0
accD_p1	accD	1	CGTTTCGGATCATGGATCGA	TTTCCCTTCGCGCAAAATTC	20	20	50.0	45.0	57.9	57.9	0.0	8.7	0.193115	88082	89797	2569-2609	1.000000	79.6
accD_p2	accD	2	CGTTTCGGATCATGGATCGA	CTGGGGGTTCTTGAGGGATA	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.240886	88082	89797	2511-2551	1.000000	79.2
accD_p3	accD	3	CGTTTCGGATCATGGATCGA	CCCTTGCCTAATTTCACGGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.256985	88082	89797	2474-2514	1.000000	79.1
accD_p4	accD	4	CGTTTCGGATCATGGATCGA	GGACCCCCTTGCCTAATTTC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.357539	88082	89797	2479-2519	1.000000	78.3
accD_p5	accD	5	CGTTTCGGATCATGGATCGA	ACCCCCTTGCCTAATTTCAC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.365918	88082	89797	2477-2517	1.000000	78.3
accD-psaI_p1	accD-psaI	1	GCTCCATGCTTTCTTTCCTT	AGAAACCATTGCAATTGCCG	20	20	45.0	45.0	56.3	57.9	0.0	26.9	1.787596	89798	90699	1027-1047	1.000000	68.2
accD-psaI_p2	accD-psaI	2	GCTCCATGCTTTCTTTCCTT	CCGGAAAAACTAAGCCCACT	20	20	45.0	50.0	56.3	58.1	0.0	0.0	1.798793	89798	90699	1010-1030	1.000000	68.1
accD-psaI_p3	accD-psaI	3	GCTCCATGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAA	20	20	45.0	45.0	56.3	58.2	0.0	26.9	1.898561	89798	90699	1022-1042	1.000000	67.3
accD-psaI_p4	accD-psaI	4	GCTCCATGCTTTCTTTCCTTT	AGAAACCATTGCAATTGCCG	21	20	42.9	45.0	57.1	57.9	0.0	26.9	2.033008	89798	90699	1027-1047	1.000000	66.2
accD-psaI_p5	accD-psaI	5	GCTCCATGCTTTCTTTCCTTT	CCGGAAAAACTAAGCCCACT	21	20	42.9	50.0	57.1	58.1	0.0	0.0	2.044204	89798	90699	1010-1030	1.000000	66.1
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.9	56.3	6.4	12.5	1.763328	95631	97061	1577-1578	1.000000	66.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	95631	97061	1500-1501	1.000000	65.5
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.883014	95631	97061	1586-1587	1.000000	65.4
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	58.2	56.3	0.0	12.5	1.895919	95631	97061	1591-1592	1.000000	65.3
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	0.0	12.5	1.899345	95631	97061	1585-1586	1.000000	65.3
ndhA_p1	ndhA	1	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	21	20	47.6	50.0	58.1	58.5	13.3	6.3	1.595303	152722	155106	2598-2609	1.000000	68.3
ndhA_p2	ndhA	2	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAAT	21	20	47.6	50.0	57.8	58.5	0.0	6.3	1.727781	152722	155106	2601-2612	1.000000	67.2
ndhA_p3	ndhA	3	ACCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	21	19	47.6	52.6	58.1	58.0	13.3	6.3	2.111750	152722	155106	2598-2609	1.000000	64.1
ndhA_p4	ndhA	4	TGAACCCAGTTACCATAGGGA	GCCCACCGGGTTTTATCAA	21	19	47.6	52.6	57.8	58.0	0.0	6.3	2.244227	152722	155106	2601-2612	1.000000	63.1
ndhA_p5	ndhA	5	CCCAGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	20	20	50.0	50.0	56.2	58.5	13.3	6.3	2.305866	152722	155106	2597-2608	1.000000	62.6