Hotspot

rps19

IRa · 156041-156319 · 279 bp

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Pi 0.00165
MarkerSeek score 34.00
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRa
length_bp
279
variable_sites
3
pi
0.00165
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 TGGACATAGAATGCCAATCT TTATCCCCCGCCAATGAATG 40.0 50.0 54.1 58.0 385-387 bp 0.923077 45.9
rps19_p2 TGGACATAGAATGCCAATCT CCCCCGCCAATGAATGATAA 40.0 50.0 54.1 58.0 381-383 bp 0.923077 45.7
rps19_p3 TGGACATAGAATGCCAATCT CATAAGGTTTATCCCCCGCC 40.0 55.0 54.1 58.1 393-395 bp 0.923077 45.2
rps19_p4 TGGACATAGAATGCCAATCTT TTATCCCCCGCCAATGAATG 38.1 50.0 55.0 58.0 385-387 bp 0.923077 44.9
rps19_p5 TGGACATAGAATGCCAATCTT CCCCCGCCAATGAATGATAA 38.1 50.0 55.0 58.0 381-383 bp 0.923077 44.7