Hotspot

rpl16

LSC · 82161-83553 · 1393 bp

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Pi 0.0035
MarkerSeek score 50.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1393
variable_sites
26
pi
0.0035
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCATCCTCAATACCCCTTTTGT ACAGTTTCTGTTTTTCCGTT 40.9 35.0 57.1 53.9 1528-1556 bp 1.0 41.8
rpl16_p2 TCATCCTCAATACCCCTTTTGTT ACAGTTTCTGTTTTTCCGTT 39.1 35.0 57.7 53.9 1528-1556 bp 1.0 41.8
rpl16_p3 TTCATCCTCAATACCCCTTTTGT ACAGTTTCTGTTTTTCCGTT 39.1 35.0 57.7 53.9 1529-1557 bp 1.0 41.8
rpl16_p4 CATCCTCAATACCCCTTTTGT ACAGTTTCTGTTTTTCCGTT 42.9 35.0 55.5 53.9 1527-1555 bp 1.0 41.8
rpl16_p5 CATCCTCAATACCCCTTTTGTT ACAGTTTCTGTTTTTCCGTT 40.9 35.0 56.3 53.9 1527-1555 bp 1.0 41.8