Hotspot

psbA

LSC · 91-1152 · 1062 bp

Pi 0.00176

MarkerSeek score 31.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 7
pi: 0.00176
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGGTATAGCTCCCTCAACGA	GTGCTTGGGAGTCCTTGAAA	55.0	50.0	58.3	58.0	1156 bp	0.923077	75.1
psbA_p2	GGGTATAGCTCCCTCAACGA	GTTTGGGGAGGGGTCTTTTT	55.0	50.0	58.3	57.9	1441-1443 bp	0.923077	74.8
psbA_p3	GGGTATAGCTCCCTCAACGA	GTATGTGTGCTTGGGAGTCC	55.0	55.0	58.3	58.3	1162 bp	0.923077	73.3
psbA_p4	TGGGTATAGCTCCCTCAACG	GTGCTTGGGAGTCCTTGAAA	55.0	50.0	58.6	58.0	1157 bp	0.923077	72.8
psbA_p5	GGGTATAGCTCCCTCAACGA	TATGTGTGCTTGGGAGTCCT	55.0	50.0	58.3	58.3	1161 bp	0.923077	72.6