Hotspot

ccsA

SSC · 115690-116658 · 969 bp

Pi 0.000767

MarkerSeek score 34.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 4
pi: 0.000767
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATACTCTAAA	AGGGTTCTCAAAAACTCGCA	50.0	45.0	57.4	57.7	1124 bp	1.0	72.9
ccsA_p2	GAGTGGCGGCATACTCTAAA	GGGTTCTCAAAAACTCGCAC	50.0	50.0	57.4	57.6	1123 bp	1.0	72.4
ccsA_p3	AGTGGCGGCATACTCTAAAA	AGGGTTCTCAAAAACTCGCA	45.0	45.0	56.9	57.7	1123 bp	1.0	69.1
ccsA_p4	AGTGGCGGCATACTCTAAAAG	AGGGTTCTCAAAAACTCGCA	47.6	45.0	58.1	57.7	1123 bp	1.0	68.9
ccsA_p5	GTGGCGGCATACTCTAAAAGA	AGGGTTCTCAAAAACTCGCA	47.6	45.0	58.1	57.7	1122 bp	1.0	68.9