Hotspot

ccsA-ndhD

SSC · 116659-116874 · 216 bp

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Pi 0.0262
MarkerSeek score 67.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
216
variable_sites
13
pi
0.0262
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCGGCACTTGTAGCTTCT GGATTTCGTTCTCTCGCTGT 45.0 50.0 57.2 58.0 393-401 bp 1.0 80.8
ccsA-ndhD_p2 ATTCGGCACTTGTAGCTTCT TTTCGTTCTCTCGCTGTCAG 45.0 50.0 57.2 57.9 390-398 bp 1.0 80.3
ccsA-ndhD_p3 ACAAATTCGGCACTTGTAGC GGATTTCGTTCTCTCGCTGT 45.0 50.0 57.0 58.0 397-405 bp 1.0 79.0
ccsA-ndhD_p4 ACAAATTCGGCACTTGTAGC TTTCGTTCTCTCGCTGTCAG 45.0 50.0 57.0 57.9 394-402 bp 1.0 78.5
ccsA-ndhD_p5 AATTCGGCACTTGTAGCTTCT GGATTTCGTTCTCTCGCTGT 42.9 50.0 57.9 58.0 394-402 bp 1.0 78.5