Hotspot

ndhF-rpl32

SSC · 113739-114692 · 954 bp

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Pi 0.00336
MarkerSeek score 53.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
954
variable_sites
16
pi
0.00336
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGCGGGAATAAGCAGAAGTC TGCAGCTAAATTTCCCTTTTTCT 50.0 34.8 58.0 57.2 1130-1157 bp 1.0 51.2
ndhF-rpl32_p2 TATTTTTGGTTGCTGCGGGA TGCAGCTAAATTTCCCTTTTTCT 45.0 34.8 58.4 57.2 1143-1170 bp 1.0 48.3
ndhF-rpl32_p3 TTTGGTTGCTGCGGGAATAA TGCAGCTAAATTTCCCTTTTTCT 45.0 34.8 58.4 57.2 1139-1166 bp 1.0 48.3
ndhF-rpl32_p4 TTTTGGTTGCTGCGGGAATA TGCAGCTAAATTTCCCTTTTTCT 45.0 34.8 58.4 57.2 1140-1167 bp 1.0 48.3
ndhF-rpl32_p5 TGCGGGAATAAGCAGAAGTC TGCAGCTAAATTTCCCTTTTTCTT 50.0 33.3 58.0 57.8 1130-1157 bp 1.0 48.3