Hotspot

ycf4-cemA

LSC · 60875-61731 · 857 bp

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Pi 0.00177
MarkerSeek score 58.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
857
variable_sites
8
pi
0.00177
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGCGTACCAATTGAAGTA ATGAGAGAGAGACCCACCAG 45.0 55.0 57.9 57.8 827-973 bp 1.0 82.8
ycf4-cemA_p2 CTTGCGCGTACCAATTGAAG ATGAGAGAGAGACCCACCAG 50.0 55.0 58.1 57.8 829-975 bp 1.0 82.5
ycf4-cemA_p3 TGCGCGTACCAATTGAAGTA GGGAGGGAAGTCAATGCTTT 45.0 50.0 57.9 57.8 780-926 bp 1.0 82.3
ycf4-cemA_p4 CTTGCGCGTACCAATTGAAG GGGAGGGAAGTCAATGCTTT 50.0 50.0 58.1 57.8 782-928 bp 1.0 82.0
ycf4-cemA_p5 GGAACCATTCCCTTGACTCG ATGAGAGAGAGACCCACCAG 55.0 55.0 58.3 57.8 909-1055 bp 1.0 81.3