Hotspot

ndhC-trnV-UAC

LSC · 50403-52007 · 1605 bp

Back to Hosta

Pi 0.00181
MarkerSeek score 52.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1605
variable_sites
15
pi
0.00181
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1710-1736 bp 1.0 79.4
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGCCCAATGCAACAAAATGC 50.0 45.0 57.9 58.5 1792-1818 bp 1.0 77.1
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1723-1749 bp 1.0 76.8
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1709-1735 bp 1.0 76.7
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.4 57.8 1727-1753 bp 1.0 75.7