Hotspot

trnT-GGU-psbD

LSC · 31543-32633 · 1091 bp

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Pi 0.00209
MarkerSeek score 58.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1091
variable_sites
13
pi
0.00209
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CGGACCAACCTACAAAAACG 55.0 50.0 58.1 57.3 941-1253 bp 1.0 78.9
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GCCGGACCAACCTACAAAAA 55.0 50.0 58.1 58.7 943-1255 bp 1.0 78.9
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA CGGACCAACCTACAAAAACG 50.0 50.0 58.1 57.3 944-1256 bp 1.0 78.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GAAAGAGCAATAGGCCGGAC 55.0 55.0 58.1 58.7 956-1268 bp 1.0 78.7
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA GCCGGACCAACCTACAAAAA 50.0 50.0 58.1 58.7 946-1258 bp 1.0 78.7