Hotspot

trnK-UUU-rps16

LSC · 4051-4580 · 530 bp

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Pi 0.00681
MarkerSeek score 51.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
530
variable_sites
12
pi
0.00681
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAA ACAAGGGAATCAATCATTCGT 45.0 38.1 58.1 55.6 618-1393 bp 1.0 53.8
trnK-UUU-rps16_p2 TACCGTTGAGTTAGCAACCC ACAAGGGAATCAATCATTCGT 50.0 38.1 57.5 55.6 593-1368 bp 1.0 51.2
trnK-UUU-rps16_p3 TAGTCGCACTTAAAAGCCGA ACAAGGGAATCAATCATTCGT 45.0 38.1 57.3 55.6 619-1394 bp 1.0 49.0
trnK-UUU-rps16_p4 GCCGAATACTCTACCGTTGA ACAAGGGAATCAATCATTCGT 50.0 38.1 57.1 55.6 604-1379 bp 1.0 48.0
trnK-UUU-rps16_p5 AGCCGAATACTCTACCGTTG ACAAGGGAATCAATCATTCGT 50.0 38.1 57.1 55.6 605-1380 bp 1.0 48.0