Hotspot

psbA-trnK-UUU

LSC · 1153-1392 · 240 bp

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Pi 0.00796
MarkerSeek score 49.80
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
240
variable_sites
6
pi
0.00796
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TAAAGACGGTTTTCGGTGCT GTTTGGGGAGGGGTCTTTTT 45.0 50.0 57.8 57.9 414-416 bp 1.0 78.8
psbA-trnK-UUU_p2 GCTTGTACTTTCGCGTCTCT GTTTGGGGAGGGGTCTTTTT 50.0 50.0 58.3 57.9 364-366 bp 1.0 78.6
psbA-trnK-UUU_p3 GTAAAGACGGTTTTCGGTGC GTTTGGGGAGGGGTCTTTTT 50.0 50.0 57.7 57.9 415-417 bp 1.0 78.3
psbA-trnK-UUU_p4 CCCCACAGGCTTGTACTTTC GTTTGGGGAGGGGTCTTTTT 55.0 50.0 58.5 57.9 372-374 bp 1.0 77.2
psbA-trnK-UUU_p5 TAAAGACGGTTTTCGGTGCT TTTGGGGAGGGGTCTTTTTC 45.0 50.0 57.8 57.6 413-415 bp 1.0 76.5