Hotspot

rrn5-ndhF

IRb · 110944-111014 · 71 bp

Back to Horsfieldia

Pi 0.0606

MarkerSeek score 67.00

Primer available yes

Label rrn5-ndhF

Feature Metadata

label_name: rrn5-ndhF
feature_type: igs
region: IRb
length_bp: 71
variable_sites: 8
pi: 0.0606
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-ndhF_p1	GGGCCTTACAATCACTAGCC	ATGGTGTTGGCATTGGAAGT	55.0	45.0	58.0	58.0	395-417 bp	1.0	83.2
rrn5-ndhF_p2	GGGGCCTTACAATCACTAGC	ATGGTGTTGGCATTGGAAGT	55.0	45.0	58.0	58.0	396-418 bp	1.0	83.2
rrn5-ndhF_p3	CCTTGTTCCTACATGACCCG	ATGGTGTTGGCATTGGAAGT	55.0	45.0	58.0	58.0	559-581 bp	1.0	82.2
rrn5-ndhF_p4	GGAGCGACAGAAGTCTTGAG	ATGGTGTTGGCATTGGAAGT	55.0	45.0	58.0	58.0	690-712 bp	1.0	82.0
rrn5-ndhF_p5	GTCAAGTGGAAGTGCAGTGA	ATGGTGTTGGCATTGGAAGT	50.0	45.0	58.0	58.0	620-642 bp	1.0	81.8