Hotspot

psbT-psbN

LSC · 77099-77157 · 59 bp

Back to Horsfieldia

Pi 0.0339

MarkerSeek score 62.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 4
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACGATCGAATCCATGGAAGC	TCGCCATCTCCATATCTGGT	50.0	50.0	58.1	58.0	292 bp	1.0	79.9
psbT-psbN_p2	CGATCGAATCCATGGAAGCA	TCGCCATCTCCATATCTGGT	50.0	50.0	58.1	58.0	291 bp	1.0	79.9
psbT-psbN_p3	ACGATCGAATCCATGGAAGC	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.1	58.1	298 bp	1.0	79.4
psbT-psbN_p4	CGATCGAATCCATGGAAGCA	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.1	58.1	297 bp	1.0	79.4
psbT-psbN_p5	ACGATCGAATCCATGGAAGC	GGGTACGCCTTATATACCGC	50.0	55.0	58.1	57.7	254 bp	1.0	78.0