Hotspot

rpoC1-rpoB

LSC · 24111-24136 · 26 bp

Back to Horsfieldia

Pi 0.0833

MarkerSeek score 71.40

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 4
pi: 0.0833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	TGGCCCAAGCACTTATTTGT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	219 bp	1.0	77.0
rpoC1-rpoB_p2	TGTTGGCCCAAGCACTTATT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	222 bp	1.0	77.0
rpoC1-rpoB_p3	TTGTTGGCCCAAGCACTTAT	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.0	57.5	223 bp	1.0	77.0
rpoC1-rpoB_p4	GGTAGGATTTTGTTGGCCCA	CCTGAGGATGCTCCAGAATC	50.0	55.0	58.1	57.5	232 bp	1.0	76.5
rpoC1-rpoB_p5	TTTGTTGGCCCAAGCACTTA	CCTGAGGATGCTCCAGAATC	45.0	55.0	58.2	57.5	224 bp	1.0	75.3