Hotspot

rpoC2-rpoC1

LSC · 21158-21319 · 162 bp

Back to Horsfieldia

Pi 0.0134

MarkerSeek score 52.20

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 4
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	AAAAACCAGATCGGCTCGTT	GGCCTACTCATACGGTACCT	45.0	55.0	58.1	58.0	211 bp	1.0	80.6
rpoC2-rpoC1_p2	AAAAACCAGATCGGCTCGTT	GTTTTGTCGGGCCTACTCAT	45.0	50.0	58.1	57.9	220 bp	1.0	79.7
rpoC2-rpoC1_p3	TGTGAAAAACCAGATCGGCT	GGCCTACTCATACGGTACCT	45.0	55.0	57.7	58.0	215 bp	1.0	79.3
rpoC2-rpoC1_p4	TGTGAAAAACCAGATCGGCT	GTTTTGTCGGGCCTACTCAT	45.0	50.0	57.7	57.9	224 bp	1.0	78.4
rpoC2-rpoC1_p5	CGTTTCATGGCAGTTGCATC	GGCCTACTCATACGGTACCT	50.0	55.0	58.4	58.0	246 bp	1.0	78.2