Hotspot

atpH-atpI

LSC · 14101-15140 · 1040 bp

Back to Horsfieldia

Pi 0.00833

MarkerSeek score 54.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 15
pi: 0.00833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1128-1162 bp	1.0	76.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CAAGCGGGATTCAAGCTCTT	45.0	50.0	58.0	58.5	1149-1183 bp	1.0	76.4
atpH-atpI_p3	CAGTACCTTGACCAACTCCG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	1187-1221 bp	1.0	76.2
atpH-atpI_p4	CAGTACCTTGACCAACTCCG	CAAGCGGGATTCAAGCTCTT	55.0	50.0	57.9	58.5	1208-1242 bp	1.0	76.0
atpH-atpI_p5	GATACCTTCTACGGCTTGGC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1213-1247 bp	1.0	75.8