Hotspot

trnS-GCU-trnG-UCC

LSC · 8428-9319 · 892 bp

Back to Horsfieldia

Pi 0.00747

MarkerSeek score 51.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 12
pi: 0.00747
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGTCGCTTTAGTCCA	AATCAAACCGAAAGACCCCC	50.0	50.0	58.0	58.1	1004-1015 bp	1.0	80.4
trnS-GCU-trnG-UCC_p2	AATCCGTCGCTTTAGTCCAC	AATCAAACCGAAAGACCCCC	50.0	50.0	58.0	58.1	1003-1014 bp	1.0	80.4
trnS-GCU-trnG-UCC_p3	CAAGTGATCAGAGGGAACGG	AATCAAACCGAAAGACCCCC	55.0	50.0	58.0	58.1	1074-1085 bp	1.0	80.3
trnS-GCU-trnG-UCC_p4	CAATCCGTCGCTTTAGTCCA	AACCGAAAGACCCCCTAACT	50.0	50.0	58.0	58.3	999-1010 bp	1.0	79.0
trnS-GCU-trnG-UCC_p5	AATCCGTCGCTTTAGTCCAC	AACCGAAAGACCCCCTAACT	50.0	50.0	58.0	58.3	998-1009 bp	1.0	79.0