Hotspot

psbI-trnS-GCU

LSC · 8201-8339 · 139 bp

Back to Horsfieldia

Pi 0.00839
MarkerSeek score 51.30
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
139
variable_sites
2
pi
0.00839
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CGTAATCCTGGACGTGAGGA TGGACTAAAGCGACGGATTG 55.0 50.0 58.9 58.0 231-236 bp 1.0 75.2
psbI-trnS-GCU_p2 CGTAATCCTGGACGTGAGGA GTGGACTAAAGCGACGGATT 55.0 50.0 58.9 58.0 232-237 bp 1.0 75.1
psbI-trnS-GCU_p3 CGTAATCCTGGACGTGAGGA TAGGAGAGATGGCTGAGTGG 55.0 55.0 58.9 57.9 248-253 bp 1.0 74.4
psbI-trnS-GCU_p4 CGTAATCCTGGACGTGAGGA CGAGTTATTCGTACCGAGGG 55.0 55.0 58.9 57.6 198-203 bp 1.0 72.4
psbI-trnS-GCU_p5 CGTAATCCTGGACGTGAGGA CTGAGTGGACTAAAGCGACG 55.0 55.0 58.9 58.4 236-241 bp 1.0 72.1