Hotspot

rps16-trnQ-UUG

LSC · 6105-7069 · 965 bp

Back to Horsfieldia

Pi 0.0159

MarkerSeek score 58.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 28
pi: 0.0159
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1034-1042 bp	1.0	81.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1038-1046 bp	1.0	80.0
rps16-trnQ-UUG_p3	CCGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1049-1057 bp	1.0	79.2
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	45.0	50.0	58.1	57.6	1040-1048 bp	1.0	78.3
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	ACTCGGAGGTTCGAATCCTT	45.0	50.0	58.1	58.4	1039-1047 bp	1.0	78.2