Hotspot

trnH-GUG-psbA

LSC · 79-377 · 299 bp

Back to Horsfieldia

Pi 0.039

MarkerSeek score 55.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 299
variable_sites: 19
pi: 0.039
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTGATGGTATGCCCCTAGGA	55.0	55.0	58.1	58.0	878-888 bp	1.0	81.1
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CCTCTGGACCTAGCTGCTAT	55.0	55.0	58.1	58.0	366-376 bp	0.75	71.7
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CCTCTGGACCTAGCTGCTAT	50.0	55.0	57.9	58.0	380-390 bp	0.75	71.6
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CCTCTGGACCTAGCTGCTAT	50.0	55.0	57.9	58.0	379-389 bp	0.75	71.6
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCTCTGGACCTAGCTGCTAT	50.0	55.0	57.9	58.0	381-391 bp	0.75	71.5