Hotspot

rbcL

LSC · 54894-56339 · 1446 bp

Back to Hordeum

Pi 0.00767

MarkerSeek score 37.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1446
variable_sites: 20
pi: 0.00767
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TTGTGGAGGATCTGTGCCTA	GACTCACGATTACGCCTGTT	50.0	50.0	58.0	58.0	2638-2666 bp	1.0	82.0
rbcL_p2	TTGTGGAGGATCTGTGCCTA	GTTGACTCACGATTACGCCT	50.0	50.0	58.0	58.0	2641-2669 bp	1.0	82.0
rbcL_p3	TTGTGGAGGATCTGTGCCTA	TTGACTCACGATTACGCCTG	50.0	50.0	58.0	58.0	2640-2668 bp	1.0	82.0
rbcL_p4	GGGATTAATTTGGGTTGCGC	GACTCACGATTACGCCTGTT	50.0	50.0	58.1	58.0	2466-2494 bp	1.0	82.0
rbcL_p5	GGGATTAATTTGGGTTGCGC	GTTGACTCACGATTACGCCT	50.0	50.0	58.1	58.0	2469-2497 bp	1.0	82.0