Hotspot

atpI

LSC · 31125-31868 · 744 bp

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Pi 0.00161

MarkerSeek score 34.10

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 3
pi: 0.00161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	AAATTGTGACCCGGATCTCG	TGGTGAGTAGTTCCTCGTGT	50.0	50.0	58.0	58.0	1709-1710 bp	1.0	81.3
atpI_p2	AAATTGTGACCCGGATCTCG	TTCTGGCTGTCTCGCAATAC	50.0	50.0	58.0	58.0	1840-1841 bp	1.0	81.2
atpI_p3	ATCAGCTGCAATAAGGGCTC	TGGTGAGTAGTTCCTCGTGT	50.0	50.0	58.0	58.0	2033-2034 bp	1.0	81.1
atpI_p4	ATCAGCTGCAATAAGGGCTC	TTCTGGCTGTCTCGCAATAC	50.0	50.0	58.0	58.0	2164-2165 bp	1.0	81.0
atpI_p5	ATCAGCTGCAATAAGGGCTC	CCCGCATAAAAGAGGACTCC	50.0	55.0	58.0	58.0	1719 bp	1.0	80.6