Hotspot

ccsA-ndhD

SSC · 107897-108062 · 166 bp

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Pi 0.0185

MarkerSeek score 47.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 166
variable_sites: 8
pi: 0.0185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGTTCGTTTACATTAACACCT	TTGACAGGGTAGAGGCTCTC	36.4	55.0	55.9	58.1	238 bp	1.0	49.1
ccsA-ndhD_p2	TGGTTCGTTTACATTAACACCT	GACAGGGTAGAGGCTCTCTT	36.4	55.0	55.9	57.8	236 bp	1.0	49.0
ccsA-ndhD_p3	TGGTTCGTTTACATTAACACCT	CCCTATCCGTTGACAGGGTA	36.4	55.0	55.9	58.2	247 bp	1.0	48.4
ccsA-ndhD_p4	TGGTTCGTTTACATTAACACCT	TCTCCCTATCCGTTGACAGG	36.4	55.0	55.9	58.2	250 bp	1.0	48.3
ccsA-ndhD_p5	GCACTTATAGCTTCGATAGGA	TTGACAGGGTAGAGGCTCTC	42.9	55.0	54.7	58.1	321 bp	1.0	46.8