Hotspot

ndhC-trnV-UAC

LSC · 50236-51140 · 905 bp

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Pi 0.00788

MarkerSeek score 51.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 905
variable_sites: 11
pi: 0.00788
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCTGAAATCGAAAATGCC	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.1	57.9	836-1040 bp	1.0	83.8
ndhC-trnV-UAC_p2	GCCCTGAAATCGAAAATGCC	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.1	58.3	837-1041 bp	1.0	81.9
ndhC-trnV-UAC_p3	CTCTGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.3	57.9	865-1069 bp	1.0	79.1
ndhC-trnV-UAC_p4	TCTGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.7	57.9	864-1068 bp	1.0	78.4
ndhC-trnV-UAC_p5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.2	57.9	855-1059 bp	1.0	78.2