Hotspot

trnF-GAA-ndhJ

LSC · 47975-48561 · 587 bp

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Pi 0.00806

MarkerSeek score 48.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 587
variable_sites: 10
pi: 0.00806
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	50.0	50.0	58.1	57.9	721-722 bp	1.0	79.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	696-697 bp	1.0	79.9
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	686-687 bp	1.0	79.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	50.0	45.0	58.1	57.5	733-734 bp	1.0	77.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	50.0	50.0	58.1	58.5	724-725 bp	1.0	76.9