Hotspot

trnS-GGA-rps4

LSC · 45039-45350 · 312 bp

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Pi 0.0139

MarkerSeek score 48.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 11
pi: 0.0139
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTCGAATCCCTCTCTTTCCG	ATAGGAAGTGGGTCGGTCTC	50.0	55.0	57.0	58.2	389-400 bp	1.0	75.0
trnS-GGA-rps4_p2	ATGTAGGAAAGATGGCCGAG	AGATAGGAAGTGGGTCGGTC	50.0	55.0	57.1	58.2	463-474 bp	1.0	74.7
trnS-GGA-rps4_p3	ATGTAGGAAAGATGGCCGAG	ATAGGAAGTGGGTCGGTCTC	50.0	55.0	57.1	58.2	461-472 bp	1.0	74.7
trnS-GGA-rps4_p4	ATGTAGGAAAGATGGCCGAG	GATAGGAAGTGGGTCGGTCT	50.0	55.0	57.1	58.2	462-473 bp	1.0	74.7
trnS-GGA-rps4_p5	ATGTAGGAAAGATGGCCGAG	GAAGTGGGTCGGTCTCAAAA	50.0	50.0	57.1	57.8	457-468 bp	1.0	74.5