Hotspot

rps15

SSC · 121354-121626 · 273 bp

Back to Hopea

Pi 0.25
MarkerSeek score 71.70
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
273
variable_sites
102
pi
0.25
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.75
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 TAGAAAAAGGGAGTCTGCGC ACCATTTCTTTGTAGCCCGG 50.0 50.0 57.9 58.2 961-975 bp 0.5 59.7
rps15_p2 TAGAAAAAGGGAGTCTGCGC CCATTTCTTTGTAGCCCGGT 50.0 50.0 57.9 58.2 960-974 bp 0.5 59.7
rps15_p3 TCCGCCATAAAAGGTCCAAG ACCATTTCTTTGTAGCCCGG 50.0 50.0 57.9 58.2 934-948 bp 0.5 59.4
rps15_p4 TCCGCCATAAAAGGTCCAAG CCATTTCTTTGTAGCCCGGT 50.0 50.0 57.9 58.2 933-947 bp 0.5 59.4
rps15_p5 TAGAAAAAGGGAGTCTGCGC GTAACCGTCTTGCTAGCGAT 50.0 50.0 57.9 57.8 1332-1346 bp 0.5 59.0