Hotspot

ndhG

SSC · 116482-117012 · 531 bp

Back to Hopea

Pi 0.303
MarkerSeek score 81.90
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
242
pi
0.303
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 AGTGCTTGCTGCTGTTAGTT ATTTTGCGGTAACTGCGTTG 45.0 45.0 58.0 58.0 1061-1063 bp 0.5 60.0
ndhG_p2 TTGAGTGCTTGCTGCTGTTA ATTTTGCGGTAACTGCGTTG 45.0 45.0 58.0 58.0 1064-1066 bp 0.5 59.9
ndhG_p3 AGTGCTTGCTGCTGTTAGTT ACGACCGTCACGAATTGAAT 45.0 45.0 58.0 57.9 987-989 bp 0.5 59.8
ndhG_p4 AGTGCTTGCTGCTGTTAGTT TCTACTTACGACCGTCACGA 45.0 50.0 58.0 57.9 994-996 bp 0.5 59.7
ndhG_p5 TTGAGTGCTTGCTGCTGTTA ACGACCGTCACGAATTGAAT 45.0 45.0 58.0 57.9 990-992 bp 0.5 59.7