Hotspot

ndhF

SSC · 107898-110141 · 2244 bp

Pi 0.239

MarkerSeek score 78.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 807
pi: 0.239
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACCCCCGTAACTTCTTCGTA	ATTGTTGTCGCAGTCCACTT	50.0	45.0	58.1	58.0	2830-2850 bp	0.5	60.3
ndhF_p2	TCGTAACCCCCGTAACTTCT	ATTGTTGTCGCAGTCCACTT	50.0	45.0	58.1	58.0	2835-2855 bp	0.5	60.3
ndhF_p3	CAATTCCCGTTCTCAACCCA	ATTGTTGTCGCAGTCCACTT	50.0	45.0	58.1	58.0	2881-2901 bp	0.5	60.1
ndhF_p4	GAGGTTTCCTTCGTAACCCC	ATTGTTGTCGCAGTCCACTT	55.0	45.0	57.9	58.0	2845-2865 bp	0.5	60.0
ndhF_p5	ATTCCCGTTCTCAACCCATG	ATTGTTGTCGCAGTCCACTT	50.0	45.0	57.9	58.0	2879-2899 bp	0.5	59.8