Hotspot

ndhA

SSC · 117904-120067 · 2164 bp

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Pi 0.26
MarkerSeek score 78.80
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2164
variable_sites
841
pi
0.26
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 ATTCAATTCGTGACGGTCGT AGAAAGGGCTGCAAAATGGA 45.0 45.0 57.9 58.0 2941-2947 bp 0.5 59.9
ndhA_p2 TCGTGACGGTCGTAAGTAGA AGAAAGGGCTGCAAAATGGA 50.0 45.0 57.9 58.0 2934-2940 bp 0.5 59.9
ndhA_p3 TTGACCAATGTACCTCGCTG AGAAAGGGCTGCAAAATGGA 50.0 45.0 57.9 58.0 2605-2611 bp 0.5 59.8
ndhA_p4 CGGCCCAAAGCAATTTGATT AGAAAGGGCTGCAAAATGGA 45.0 45.0 57.9 58.0 2964-2970 bp 0.5 59.8
ndhA_p5 ATTCAATTCGTGACGGTCGT AGGGCTGCAAAATGGAATGA 45.0 45.0 57.9 58.1 2937-2943 bp 0.5 59.6