Hotspot

ccsA

SSC · 112567-113532 · 966 bp

Back to Hopea

Pi 0.242
MarkerSeek score 78.70
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
966
variable_sites
351
pi
0.242
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.78
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TGGAATTGGTAGACACGCTG ACTATCAGGTGAGAGGGTCG 50.0 55.0 57.9 57.9 1459 bp 0.5 59.4
ccsA_p2 GTGGAATTGGTAGACACGCT ACTATCAGGTGAGAGGGTCG 50.0 55.0 57.9 57.9 1460 bp 0.5 59.4
ccsA_p3 TAGACACGCTGCTCTTAGGA ACTATCAGGTGAGAGGGTCG 50.0 55.0 57.9 57.9 1450 bp 0.5 59.2
ccsA_p4 TGGAATTGGTAGACACGCTG CGGCTTGTTTATCTCGACGA 50.0 50.0 57.9 58.1 1842 bp 0.5 59.1
ccsA_p5 GTGGAATTGGTAGACACGCT CGGCTTGTTTATCTCGACGA 50.0 50.0 57.9 58.1 1843 bp 0.5 59.0