Hotspot

rps15-trnN-GUU

SSC · 121627-128037 · 6411 bp

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Pi 0.244
MarkerSeek score 73.50
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6411
variable_sites
2318
pi
0.244
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.82
markerseek_score
73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 CCAAAAGTTGCCAAACTGGG TTTTGCGGGACAGCTCATAA 50.0 45.0 58.0 57.8 1116-1200 bp 0.5 62.8
rps15-trnN-GUU_p2 CCAAAAGTTGCCAAACTGGG TTTTTGCGGGACAGCTCATA 50.0 45.0 58.0 57.8 1117-1201 bp 0.5 62.8
rps15-trnN-GUU_p3 ATGGTCGTCTCCTTCGGTTA TTTTGCGGGACAGCTCATAA 50.0 45.0 58.2 57.8 983-1067 bp 0.5 62.0
rps15-trnN-GUU_p4 TGGTCGTCTCCTTCGGTTAT TTTTGCGGGACAGCTCATAA 50.0 45.0 58.2 57.8 982-1066 bp 0.5 62.0
rps15-trnN-GUU_p5 TGGTCGTCTCCTTCGGTTAT TTTTTGCGGGACAGCTCATA 50.0 45.0 58.2 57.8 983-1067 bp 0.5 62.0