Hotspot

ndhG-ndhI

SSC · 117013-117317 · 305 bp

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Pi 0.322
MarkerSeek score 74.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
305
variable_sites
147
pi
0.322
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AGTGCTTGCTGCTGTTAGTT CAAGATGGACTTTCCCTCGG 45.0 55.0 58.0 58.0 1579-1581 bp 0.5 60.1
ndhG-ndhI_p2 AATAAGTAACTGGGCAGCGG CAAGATGGACTTTCCCTCGG 50.0 55.0 58.0 58.0 1206-1208 bp 0.5 60.1
ndhG-ndhI_p3 TTGAGTGCTTGCTGCTGTTA CAAGATGGACTTTCCCTCGG 45.0 55.0 58.0 58.0 1582-1584 bp 0.5 60.0
ndhG-ndhI_p4 AGTGCTTGCTGCTGTTAGTT ATTTTGCGGTAACTGCGTTG 45.0 45.0 58.0 58.0 1061-1063 bp 0.5 60.0
ndhG-ndhI_p5 TTGAGTGCTTGCTGCTGTTA ATTTTGCGGTAACTGCGTTG 45.0 45.0 58.0 58.0 1064-1066 bp 0.5 59.9