Hotspot

ccsA-ndhD

SSC · 113533-113784 · 252 bp

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Pi 0.214
MarkerSeek score 71.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
252
variable_sites
80
pi
0.214
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.73
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTTGCTACTTCGGGCCTTTT CTCTTTTCCCCTTGGTTGCT 45.0 50.0 58.0 58.0 1566 bp 0.5 60.5
ccsA-ndhD_p2 TTTGCTACTTCGGGCCTTTT TCTCTTTTCCCCTTGGTTGC 45.0 50.0 58.0 58.0 1567 bp 0.5 60.4
ccsA-ndhD_p3 TCCGCAACATTAGTACCAGC CTCTTTTCCCCTTGGTTGCT 50.0 50.0 58.0 58.0 1530 bp 0.5 60.4
ccsA-ndhD_p4 TCCGCAACATTAGTACCAGC TCTCTTTTCCCCTTGGTTGC 50.0 50.0 58.0 58.0 1531 bp 0.5 60.4
ccsA-ndhD_p5 CCGCAACATTAGTACCAGCT CTCTTTTCCCCTTGGTTGCT 50.0 50.0 58.0 58.0 1529 bp 0.5 60.4