Hotspot

rpl32-trnL-UAG

SSC · 111028-112367 · 1340 bp

Back to Hopea

Pi 0.255
MarkerSeek score 80.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1340
variable_sites
508
pi
0.255
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.84
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGTGGACTGCGACAACAAT AAAAGGCCCGAAGTAGCAAA 45.0 45.0 58.0 58.0 2355-2382 bp 0.5 61.6
rpl32-trnL-UAG_p2 AAGTGGACTGCGACAACAAT GCTGGTACTAATGTTGCGGA 45.0 50.0 58.0 58.0 2391-2418 bp 0.5 61.6
rpl32-trnL-UAG_p3 AAGTGGACTGCGACAACAAT AGCTGGTACTAATGTTGCGG 45.0 50.0 58.0 58.0 2392-2419 bp 0.5 61.5
rpl32-trnL-UAG_p4 AAGTGGACTGCGACAACAAT AACCATCGGGATTGAAGAGC 45.0 50.0 58.0 58.0 2409-2436 bp 0.5 61.4
rpl32-trnL-UAG_p5 AAGTGGACTGCGACAACAAT CGGGATTGAAGAGCTGGTAC 45.0 55.0 58.0 58.1 2403-2430 bp 0.5 61.3