Hotspot

ndhF-rpl32

SSC · 110142-110853 · 712 bp

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Pi 0.246
MarkerSeek score 79.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
712
variable_sites
255
pi
0.246
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.81
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAAAGCCCACATACGACGAA AAAAAGAATCCCCATCCCGG 45.0 50.0 57.8 57.8 1160-1200 bp 0.5 60.7
ndhF-rpl32_p2 AAAGCCCACATACGACGAAA AAAAAGAATCCCCATCCCGG 45.0 50.0 57.8 57.8 1159-1199 bp 0.5 60.7
ndhF-rpl32_p3 AAGCCCACATACGACGAAAA AAAAAGAATCCCCATCCCGG 45.0 50.0 57.8 57.8 1158-1198 bp 0.5 60.7
ndhF-rpl32_p4 AAGCCCACATACGACGAAAA CAAAGCTTTCAACGCTGTCC 45.0 50.0 57.8 58.2 901-932 bp 0.5 59.9
ndhF-rpl32_p5 GGTCGTGTGAACCAAAAACC AAAAAGAATCCCCATCCCGG 50.0 50.0 57.8 57.8 1540-1580 bp 0.5 59.7