Hotspot

rps11

LSC · 75743-76174 · 432 bp

Back to Homolepis

Pi 0.00231

MarkerSeek score 46.20

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 432
variable_sites: 1
pi: 0.00231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	GTGTTTGGGTGGATCCTGTT	ATGACACCATCATCCTTGGC	50.0	50.0	58.0	57.9	1123 bp	1.0	79.6
rps11_p2	GTGTTTGGGTGGATCCTGTT	TTATGATTCCAGCAAGGGGC	50.0	50.0	58.0	57.9	1029 bp	1.0	79.5
rps11_p3	GCAAGGCAATACCGACAGTA	ATGACACCATCATCCTTGGC	50.0	50.0	58.0	57.9	1234 bp	1.0	79.5
rps11_p4	GCAAGGCAATACCGACAGTA	TTATGATTCCAGCAAGGGGC	50.0	50.0	58.0	57.9	1140 bp	1.0	79.4
rps11_p5	GTGTTTGGGTGGATCCTGTT	ATTCGAAGCGTACCGAAGAC	50.0	50.0	58.0	58.1	982 bp	1.0	79.4