Hotspot

psaA

LSC · 40345-42597 · 2253 bp

Back to Homolepis

Pi 0.000888

MarkerSeek score 56.20

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 2
pi: 0.000888
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	GCTAAGCCTTGGCTAAACCT	TTGGCGGGTCTCTTTGTATG	50.0	50.0	57.9	57.9	2361 bp	1.0	78.2
psaA_p2	GCTAAGCCTTGGCTAAACCT	GTTGGCGGGTCTCTTTGTAT	50.0	50.0	57.9	57.9	2362 bp	1.0	78.2
psaA_p3	AATACGACGAGTAGTGGGGT	TTGGCGGGTCTCTTTGTATG	50.0	50.0	57.9	57.9	2386 bp	1.0	78.1
psaA_p4	AATACGACGAGTAGTGGGGT	GTTGGCGGGTCTCTTTGTAT	50.0	50.0	57.9	57.9	2387 bp	1.0	78.1
psaA_p5	CCTTGGCTAAACCTGGGAAA	TTGGCGGGTCTCTTTGTATG	50.0	50.0	57.7	57.9	2355 bp	1.0	76.8