Hotspot

trnL-UAG-ccsA

SSC · 107199-107275 · 77 bp

Back to Homolepis

Pi 0.013
MarkerSeek score 60.00
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
77
variable_sites
1
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA ACGTAATCCCCTAAGTTCACG 50.0 47.6 57.9 57.5 252 bp 1.0 67.2
trnL-UAG-ccsA_p2 TTAGCCGCCATGGTGAAATT ACGTAATCCCCTAAGTTCACG 45.0 47.6 58.2 57.5 274 bp 1.0 66.9
trnL-UAG-ccsA_p3 AAATTGGTAGACACGCTGCT ACGTAATCCCCTAAGTTCACG 45.0 47.6 57.8 57.5 259 bp 1.0 66.7
trnL-UAG-ccsA_p4 TTAGGAAGCAGTGCTCAAGC ACGTAATCCCCTAAGTTCACG 50.0 47.6 58.2 57.5 238 bp 1.0 66.7
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG ACGTAATCCCCTAAGTTCACG 55.0 47.6 57.8 57.5 253 bp 1.0 66.6