Hotspot

ndhF-rpl32

SSC · 105634-106332 · 699 bp

Back to Homolepis

Pi 0
MarkerSeek score 59.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
699
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTGGCAGAAGAGGGATTACC TCCAAAGATTTTTACGAATACGCT 55.0 33.3 57.7 57.3 800-806 bp 1.0 40.7
ndhF-rpl32_p2 GTGGCAGAAGAGGGATTACC TGACATCGAAGTACGTTTTT 55.0 35.0 57.7 53.2 772-778 bp 1.0 40.7
ndhF-rpl32_p3 GTGGCAGAAGAGGGATTACC TTGACATCGAAGTACGTTTT 55.0 35.0 57.7 53.2 773-779 bp 1.0 40.7
ndhF-rpl32_p4 GTGGCAGAAGAGGGATTACC TTTGACATCGAAGTACGTTT 55.0 35.0 57.7 53.2 774-780 bp 1.0 40.7
ndhF-rpl32_p5 GTGGCAGAAGAGGGATTACC TTTTGACATCGAAGTACGTT 55.0 35.0 57.7 53.2 775-781 bp 1.0 40.7