Hotspot

rps3-rpl22

LSC · 80160-80219 · 60 bp

Back to Homolepis

Pi 0.0167

MarkerSeek score 62.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 1
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GTGCGAACCAAAAGGAATGG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.9	57.7	229 bp	1.0	77.5
rps3-rpl22_p2	ACCAAGTCTGAAACCGAGTG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.8	57.7	195 bp	1.0	76.2
rps3-rpl22_p3	CCAAGTCTGAAACCGAGTGG	AAATTCAGACCTCGAGCTCG	55.0	50.0	58.5	57.7	194 bp	1.0	74.2
rps3-rpl22_p4	GTGCGAACCAAAAGGAATGG	CGAGCTCGAGGACGTAGTTA	50.0	55.0	57.9	58.7	217 bp	1.0	74.1
rps3-rpl22_p5	CAAGTCTGAAACCGAGTGGA	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.5	57.7	193 bp	1.0	73.9